dykddddk peptide NH2-DYKDDDDK-COOH - ThepeptideStore Peptide

DYKDDDDKTag The DYKDDDDK peptide, often referred to as the FLAG peptide, is a crucial tool in molecular biology and protein research.The FLAG peptideis used in many different assayssuch as purification, conjugation by ELISA, flow cytometry, immunoprecipitation etc. This short, highly charged peptide sequence, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, serves as a versatile fusion tag, enabling researchers to purify, detect, and study recombinant proteins. Its widespread adoption is due to its ability to facilitate efficient protein handling under various experimental conditions3xDYKDDDDKTag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available..

Understanding the DYKDDDDK Peptide and its Applications

The primary utility of the DYKDDDDK peptide lies in its role as an epitope tag. When appended to a protein of interest, either at the N-terminus, C-terminus, or within the protein sequence, it allows for specific recognition by antibodiesDYKDDDDK peptideis a synthetic octapeptide and fusion tag. ... It can be added to the N- or C-terminus of proteins or inserted into the protein sequence and .... This specific recognition is the cornerstone of many biochemical techniques.

One of the most common applications is affinity purificationRest of World. Currency: GBP; EUR. £102.00. add to basket. Estimated delivery Thu 25 Dec Shipping is calculated in checkout. Product details. CAS Number: 402750 .... Antibodies targeting the DYKDDDDK tag can be immobilized on a solid support, such as agarose beads or magnetic beads.Alexa Fluor™ 647-DYKDDDDK Peptide 0.4 mL | Buy Online When a cell lysate containing a DYKDDDDK-tagged protein is applied, the antibody binds to the tag, effectively capturing the target protein while other cellular components are washed away3X DYKDDDDK Peptide - Bachem Products. This process is instrumental for isolating proteins for further analysis.

Crucially, elution of the purified protein can be achieved under non-denaturing conditions. This is often accomplished using a free DYKDDDDK peptide in excess. The free peptide competes with the tagged protein for binding to the antibody, allowing the release of the intact, functional protein. This is particularly important for proteins that are sensitive to harsh elution conditions. For instance, efficient elution of DYKDDDDK-tagged SUMO protein can be achieved using this method, preserving the protein's structure and activitySequence ·NH2-DYKDDDDK-COOH; Molecular Weight · 1012.96 Da ; Source · Synthetic ; Format · Lyophilized white powder ; Storage (short term): 2-8°C (up to 6 months).

The 3x FLAG peptide is a common variant, featuring three copies of the DYKDDDDK sequence.DYKDDDDK (FLAG) peptide This multimeric form can offer enhanced binding affinity to the antibody, potentially leading to more efficient capture and elution.3xDYKDDDDKTag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. This 3x DYKDDDDK Tag Peptide is a polypeptide composed of 23 amino acids and has a molecular weight of 2864 Da.DYKDDDDK Peptide Similarly, the 3xFLAGpeptide is widely used for competitive elution of 3X FLAG fusion proteins.

Key Properties and Specifications

The DYKDDDDK peptide is a synthetic octapeptide. Its sequence, NH2-DYKDDDDK-COOH, has a molecular weight of approximately 1012.It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide ...96 Da in its basic form. It is typically supplied as a lyophilized white powder, which should be stored at 2-8°C for short-term storage (up to 6 months) or at -5 to -30°C for longer periods.Rest of World. Currency: GBP; EUR. £102.00. add to basket. Estimated delivery Thu 25 Dec Shipping is calculated in checkout. Product details. CAS Number: 402750 ... Purity is often specified, with many commercially available peptides being ≥95% (HPLC).

Beyond purification, the DYKDDDDK peptide is valuable in a range of other assays.Alexa Fluor™ 647-DYKDDDDK Peptide 0.4 mL | Buy Online These include ELISA, flow cytometry, and immunoprecipitationA working concentration of 500 μg/mL for triple FLAG peptideis typically used for competitive elution of FLAG containing proteinsfrom anti-DYKDDDDK affinity .... Its presence can be confirmed using specific antibodies like the M1 or M2 monoclonal antibodies, which are designed to bind the DYKDDDDK epitope.

Variations and Related Products

The DYKDDDDK tag is not limited to its simple octapeptide form. Variations exist to optimize performance or for specific applications.DYKDDDDKFab-Trap™ was used for immunoprecipitation of Flag®-tagged protein from HEK293T cell lysate. During elution with 3xDYKDDDDK-peptide... The 3X DYKDDDDK Peptide is a prominent example.Pierce™ 3x DYKDDDDK Peptide 5 mg | Buy Online The 3x DYKDDDDK-tag peptide, also known as 3x FLAG peptide, is a 23 amino acid length peptide used for the competitive elution of DYKDDDDK-tag fusion proteins with an excess of free peptideDYKDDDDK (FLAG®) Peptide (000-000-383).

Researchers may also encounter modified versions, such as the Alexa Fluor™ 647-DYKDDDDK Peptide, which can serve as a control reagent in fluorescence-based assaysThe 3xFLAG®Peptideis a 23-merpeptidefor competitive elution of 3xFLAG® tagged proteins, with the classic FLAG® sequenceDYKDDDDKat the C-terminus.. The poly DYKDDDDK Tag Peptide is another form available commercially3X DYKDDDDK Peptide.

The DYKDDDDK sequence itself also contains an enterokinase-cleavage site, which can be leveraged in certain experimental designs to remove the tag after purification if necessary.DYKDDDDK tag Peptideis a 8 amino acid peptide with an enterokinase-cleavage site used for the purification of recombinant proteins. Targets, anti-DYKDDDDK M2 ... This feature makes the DYKDDDDK tag Peptide a versatile fusion tag for protein research.

Technical Considerations and Best Practices

When using the DYKDDDDK peptide for elution, a typical working concentration for the triple FLAG peptide is around 500 µg/mL. This concentration is generally sufficient for effectively eluting FLAG containing proteins from anti-DYKDDDDK affinity matrices.

The DYKDDDDK peptide is an essential tool for researchers in various fields, including proteomics, structural biology, and cell biology. Its ability to facilitate purification, conjugation, and detection of proteins makes it indispensable for understanding protein function and localization, especially when antibodies against the native protein are not readily available. The DYKDDDDK (FLAG®) Peptide is a reliable reagent for various research needs, including SDS-PAGE analysis, and is ideal for immunoaffinity chromatography. The FLAG peptide is used in many different assays, highlighting its broad applicability.