c myc peptide c-Myc peptide
c myc peptide c-Myc Tag peptides are substitutes for c-Myc fusion proteins - Myc peptidesequence c-Myc peptide Unveiling the Utility of c-Myc Peptide in Research and Diagnostics
Peptidedirectory The c-Myc peptide is a synthetic molecule that has emerged as a valuable tool in various biological research and diagnostic applications. Derived from the c-myc gene product, this polypeptide protein tag plays a crucial role in understanding and manipulating proteins tagged with the myc tag. Its primary utility lies in its ability to interact with antibodies specifically designed to recognize the myc epitope, making it indispensable for techniques like immunoassays and protein purification.
One of the key applications of the c-Myc peptide is its role in immunoassays. It is useful for displacement of c-Myc-tagged fusion proteins bound to anti-c-Myc antibodies. This means that when an antibody, such as the widely used 9E10 antibody, is bound to a protein engineered with a c-Myc tag (often the sequence EQKLISEEDL), the addition of free c-Myc peptide can effectively compete for antibody binding. This competitive binding allows researchers to quantify the amount of tagged protein present or to disrupt existing antibody-protein interactions.c-Myc Peptide The c-Myc peptide epitope is recognized by antibodies like the Anti-c-myc antibody 9E10, which derives from the human proto-oncogene p62c-myc. This specific interaction is fundamental to many detection and purification strategies.
Beyond its use in displacement assays, the c-Myc peptide is also instrumental in the purification of c-Myc-tagged proteins.Description. The c-myc tagis often used as a protein modificationin order to simplify the labeling and detection of proteins. Antibodies against c-myc ... For instance, it may be used to elute c-Myc-tagged proteins from affinity chromatography columns, such as those utilizing Thermo Scientific Pierce Anti-c-Myc Agarose. These specialized resins are designed to capture proteins that have been modified with the c-Myc tag, facilitating their isolation from complex biological mixtures. Once the tagged protein is bound to the c-Myc tag Peptide immobilized on the resin, the addition of a soluble c-Myc peptide can disrupt the antibody-protein interaction, releasing the purified protein. This process is crucial for obtaining highly pure protein samples for further analysis or functional studies. c-Myc Tag peptides are substitutes for c-Myc fusion proteins in these competitive binding scenarios.Affinity-selected peptide ligands specifically bind i-motif DNA ...
The importance of c-Myc itself in biological processes cannot be overstatedc-Myc tag peptide (EQKLISEEDL). c-Myc is a key regulator of cell growth, and its dysregulation is implicated in a significant percentage of human cancers, with reports suggesting it is dysregulated in ~70% of cancers.145646-22-6・c-Myc Peptide・133-19211[Detail Information] As a nuclear phosphoprotein, c-Myc plays a fundamental role in cell cycle progression, apoptosis, and cellular transformation. Its function as a transcription factor highlights its central role in cellular processes. Understanding the intricate functions of c-Myc is vital for developing targeted therapies.Thec-Myc tag is a 10-residue peptide sequencecorresponding to the C-terminal sequence of human c-Myc protein, and is one of the tags used as an affinity tag ... The c-Myc peptide serves as a valuable tool in this endeavor, enabling researchers to probe the interactions of c-Myc and its associated proteins.
The c-Myc tag is a 10-residue peptide sequence derived from the C-terminal end of the human c-myc protein.c-Myc Peptide Tag from BioLegend This sequence, often represented as EQKLISEEDL, is a common addition to recombinant proteins through recombinant DNA technology. The c-Myc tag is often used as a protein modification to simplify the labeling and detection of proteins.The peptideis useful for competing out anti-c-Myc antibody bindingto c-Myc-tagged fusion proteins in immunoassays. The successful inhibition of antibody. Its small size and high specificity for anti-myc antibodies make it an attractive choice for researchers across various disciplines. The Myc-tag is therefore a versatile tool for protein characterization, enabling applications such as immunoprecipitation and Western blotting.
The c-Myc peptide can be supplied in various forms, including as a lyophilized powder or in solution, such as c-Myc Peptide (TFA). The TFA form indicates that the peptide is supplied as a trifluoroacetate salt, a common counterion for synthetic peptides. These synthetic peptides, such as the Human c-Myc peptide, are synthesized to high purity levels, often exceeding 97%, ensuring reliable and reproducible results in experimental settings作者：D Guneri·2025—c-Myc is an oncogene that is dysregulated in ∼70% of cancers. Its multifaceted function complicates effective drug targeting of the protein. i-Motif (iM) DNA .... The availability of c-Myc peptide from various suppliers, including Pierce, GenScript peptides, and BioLegend, underscores its widespread adoption in the scientific community.作者：TA Ting·2020·被引用次数：13—The transcription factorc-Myc is a key regulator of cell growthand its expression levels are tightly regulated. This might explain why its ... Researchers can find c-Myc peptide through various peptides research chemicals catalogs and order peptides online.
In summary, the c-Myc peptide is a critical reagent for researchers working with proteins engineered with a c-Myc tag.Human c-Myc peptide (ab166837) Its ability to competitively bind anti-c-Myc antibodies makes it invaluable for immunoassays, protein purification, and the broader study of c-Myc, a crucial oncogene involved in cell growth and implicated in numerous cancers. The c-Myc protein itself, a helix-loop-helix leucine zipper phosphoprotein, regulates gene transcription, and understanding its mechanisms is a key area of biological research.